The substrate-protease pair is coexpressed at stoichiometric amounts inside a 2A-based bicistronic vector, amenable to genetic manipulation. assay can probe PCSK9 proteolysis under conditions of either genetic or molecular perturbation inside a high-throughput manner. This system is definitely well suited for both the biochemical evaluation of clinically found out missense … Continue reading The substrate-protease pair is coexpressed at stoichiometric amounts inside a 2A-based bicistronic vector, amenable to genetic manipulation